Cerebral microvascular and parenchymal phospholipid composition in the mouse |
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Authors: | Wesley M Williams PhD Melvin Reichman Thomas H McNeill |
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Institution: | (1) Department of Neurology and the Center for Brain Research, University of Rochester Medical Center, Rochester, New York, USA;(2) Department of Pharmacology, University of Rochester Medical Center, 601 Elmwood Avenue, 14642 Rochester, NY |
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Abstract: | Cerebral microvessels consisting predominantly of capillaries and small arterioles (<30 m dia.) were isolated from the cerebral cortex and cerebellum of 3-month-old mice. Lipids were extracted from both microvascular and brain parenchymal fractions and the major phospholipid classes (choline phosphoglyceride, ethanolamine phosphoglyceride, inositol phosphoglyceride, serine phosphoglyceride, and sphingomyelin) separated by 2-dimensional TLC. Comparison of mol % determined by phosphate analysis of each phospholipid revealed significant differences in membrane composition of ethanolamine phosphoglyceride, inositol phosphoglyceride, and sphingomyelin between microvascular and parenchymal components of the central nervous system. Moreover, the choline phosphoglyceride/sphingomyelin mol ratio, one of three determinants of membrane fluidity, is significantly lower for microvessel membrane than for membranes of the brain parenchyma. |
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Keywords: | Cerebral microvessels lipids phospholipids |
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