首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Functional patterns and temperature response of cellulose-fermenting microbial cultures containing different methanogenic communities
Authors:Wu X L  Chin K J  Stubner S  Conrad R
Institution:Max-Planck-Institut für terrestrische Mikrobiologie, Marburg, Germany.
Abstract:The effect of microbial composition on the methanogenic degradation of cellulose was studied using two lines of anaerobic cellulose-fermenting methanogenic microbial cultures at two different temperatures: that at 15 degrees C being dominated by Methanosaeta and that at 30 degrees C by Methanosarcina. In both cultures, CH4 production and acetate consumption were completely inhibited by either 2-bromoethanesulfonate or chloroform, whereas H2 consumption was only inhibited by chloroform, suggesting that homoacetogens utilized H2 concomitantly with methanogens. Hydrogen was the intermediate that was consumed first, while acetate continued to accumulate. At 15 degrees C, acetoclastic methanogenesis smoothly followed H2-dependent CH4 production. Fluorescence in situ hybridization showed that populations of Methanosaeta steadily increased with time from 5 to 25% of total cell counts. At 30 degrees C, two phases of CH4 production were obtained, with acetate consumed after the abrupt increase of Methanosarcina from 0 to 45% of total cell counts. Whereas populations of Methanosaeta were able to adapt after transfer from 15 to 30 degrees C, those of Methanosarcina were not, irrespective of during which phase the cultures were transferred from 30 degrees C to 15 degrees C. Our results thus show that the community structure of methanogens indeed affects the function of a cellulose-fermenting community with respect to temperature response.
Keywords:
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号