Characterization of pyrophosphate exchange by the reconstituted adenine nucleotide translocator from mitochondria |
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| Authors: | Reinhard Kramer |
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| Affiliation: | Institut für Physikalische Biochemie der Universität München Goethestrasse 33, 8000 München 2, FRG |
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| Abstract: | The transport of inorganic pyrophosphate (PPi) by the adenine nucleotide translocator from beef heart mitochondria was studied in a reconstituted system. The transport of PPi is dependent on appropriate transmembrane substrates. The activity of PPi exchange is about one tenth as compared to the ADP/ATP exchange, whereas the transport affinity for PPi is very low (2-5 mM). The adenine nucleotide carrier catalyzes a strict counterexchange of PPi and nucleotides with an exchange stoichiometry close to 1. The inhibitor specificity of PPi exchange is comparable to that of ADP/ATP exchange. |
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| Keywords: | AdN adenine nucleotides AdN-T adenine nucleotide translocator BKA bongkrekate CAT carboxyatractylate orthophosphate inorganic pyrophosphate |
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