Regulation and role in pathogenicity of Erwinia chrysanthemi 3937 pectin methylesterase. |
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| Authors: | M Boccara and V Chatain |
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| Institution: | Laboratoire de Pathologie Végétale, Institut National Agronomique, Paris, France. |
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| Abstract: | The gene pem, encoding the pectin methylesterase (PME) of Erwinia chrysanthemi 3937, was cloned and mutagenized by mini-Mu transposable elements. A second gene, pecY, which could act as a negative regulator of PME was found 5' to the pem gene. A PME-E. chrysanthemi derivative inoculate onto Saintpaulia plants was shown to be clearly noninvasive, demonstrating the important role of this enzyme in soft rot disease. |
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