Physical mapping of the mitochondrial DNA from Aspergillus niger |
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Authors: | Kohtaro Kirimura Satoshi Fukuda Hidetoshi Abe Shinji Kanayama Shoji Usami |
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Affiliation: | Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo, Japan;Nippon Gene Company, Toyama Laboratory, Toyama, Japan |
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Abstract: | Abstract The mitochondrial DNA was isolated from Aspergillus niger WU-2223L, a citric acid-production strain, and characterized by restriction-endonuclease mapping. Cloned fragments which covered the total range of the mitochondrial DNA were assembled and utilized to construct the restriction-endonuclease map for nine restriction enzymes. This map showed that the mitochondrial DNA was a circular molecule of 32.6 kb. |
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Keywords: | Aspergillus niger Mitochondrial DNA Restriction mapping |
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