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Physical mapping of the mitochondrial DNA from Aspergillus niger
Authors:Kohtaro Kirimura  Satoshi Fukuda  Hidetoshi Abe  Shinji Kanayama  Shoji Usami
Institution:Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo, Japan;Nippon Gene Company, Toyama Laboratory, Toyama, Japan
Abstract:Abstract The mitochondrial DNA was isolated from Aspergillus niger WU-2223L, a citric acid-production strain, and characterized by restriction-endonuclease mapping. Cloned fragments which covered the total range of the mitochondrial DNA were assembled and utilized to construct the restriction-endonuclease map for nine restriction enzymes. This map showed that the mitochondrial DNA was a circular molecule of 32.6 kb.
Keywords:Aspergillus niger            Mitochondrial DNA  Restriction mapping
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