Inhibition of DNA polymerase alpha by bleomycin |
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Authors: | G FitzGerald M M Wick |
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Affiliation: | 1. Division of Medicine, Sidney Farber Cancer Institute-Department of Dermatology, Harvard Medical School, Boston, Massachusetts 02115 USA;2. Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115 USA |
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Abstract: | Bleomycin is an important anti-tumor agent which works primarily through it's degradation of DNA template. Using synthetic single (poly[dA]-oligo-[dT]) and double stranded (poly[dA-dT]) templates, we noted significant inhibition when the BLM resistant homopolymer was used. Furthermore, when each of the components of the DNA polymerase assay were treated with bleomycin separately, followed by removal of bleomycin, significant inhibition (35%) of the enzyme was observed. The limited inhibition of DNA polymerase by BLM was attributed to residual activity of the enzyme-inhibitor complex. |
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