Immunocytochemical detection of enhanced expression of c-myc protein in the heart of cardiomyopathic hamster |
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Authors: | Fujitani Noboru Kawaguchi Naomasa Toda Shuji Matsumura Sueo Kimura Hiroshi Onishi Shunzo |
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Institution: | (1) Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, 830, Japan;(2) School of Allied Health Sciences, Faculty of Medicine, Osaka University, Osaka, 560, Japan;(3) Department of Pathology, Saga Medical School, Saga, 849, Japan;(4) Department of Biochemistry, Saga Medical School, Saga, 849, Japan |
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Abstract: | An immunocytochemical study was performed to examine the expression of cellular c-myc protein in the heart of 30-, 120- and 180-day-old cardiomyopathic Syrian UM-X7.1 hamsters. The heart of age- and sex-matched BIO-RB hamster was used as normal control. In paraffin sections, an immunostaining for c-myc was markedly increased in cytoplasm of cells from the UM-X7.1 heart as compared with that of the BIO-RB heart which showed a weak staining. However, c-myc was localized in nuclei of cells in frozen sections of the heart. Specific cell types of the heart were differentiated with anti-vimentin, and we found that the increased expression of c-myc was present in nuclei of muscle cells of the UM-X7.1 myocardium. A quantitative study of c-myc-positive nuclei of muscle and nonmuscle cells was carried out by a video micrometer. The mean number of c-myc-positive nuclei of muscle cells was significantly higher in the cardiomyopathic heart than in the control heart from hamsters of all ages studied. These results suggest that the increase of c-myc protein may relate to the pathological state or pathogenesis of the hereditary cardiomyopathy. |
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Keywords: | c-myc cardiomyopathy hamster immunohistochemistry |
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