Autonomous Replication of a Bombyx moriTransgene Fragment in the Yeast Saccharomyces cerevisiae |
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| Authors: | Legchilina S P Efimenko I G Aniskina Yu V Arman I P Tarantul V Z Nikolaev A I |
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| Institution: | (1) Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia |
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| Abstract: | A previously cloned autonomous transgene (pr8a) of silkworm Bombyx moriinherited without changes in the structure was used to clarify the activity of its ARS in yeast cells. ARS of pr8a was also shown to maintain autonomous replication of hybrid plasmids in yeast cells. The same was true for its central 2.4-kb fragment devoid of flanking sequences. |
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