Flow cytometric analysis of nuclear genome of the Ethiopian cereal tef [Eragrostis tef (Zucc.) Trotter] |
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| Authors: | Mulu Ayele J Dole?e M Van Duren H Brunner F J Zapata-Arias |
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| Institution: | (1) Debre Zeit Agricultural Research Center, Alemaya University of Agriculture, PO Box 32, Debre Zeit, Etniopia;(2) Norman Borlaug Center for Plant Science, Institute of Experimental Botany, Department of Plant Biotechnology, De Montfort University, Sokolovska 6, CZ-77200 Olomouc, Czech Republic;(3) Plant Breeding Unit, International Atomic Energy Agency, A-2444 Seibersdorf, Austria;(4) Present address: Plant Molecular Genetics Laboratory, Texas Tech University, Mail Stop 2122, 79409 Lubbock, TX, USA |
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| Abstract: | Flow cytometric analysis of nuclear DNA content was performed by using nuclei isolated from young leaf tissue of tef (Eragrostis tef). The method was very useful for rapid screening of ploidy levels in cultivars and lines of tef representing the phenotypic variability of this species in Ethiopia. The results of the analysis showed that all cultivars were tetraploid. Flow cytometry was also used to determine nuclear DNA content in absolute units (genome size) in four tef cultivars. Nuclei isolated from tomato (Lycopersicon esculentum, 2C=1.96 pg) were used as an internal reference standard. The 2C DNA content of individual tef cultivars ranged from 1.48 to 1.52 pg (1C genome size: 714 Mbp-733 Mbp), the differences among them being statistically nonsignificant. The fact that the nuclear genome of tef is only about 50% larger than that of rice should make it amenable for analysis and mapping at the molecular level. |
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| Keywords: | DNA content Eragrostis tef (Tef) ploidy |
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