Reproductive function for a C-terminus extended, male-transmitted cytochrome c oxidase subunit II protein expressed in both spermatozoa and eggs |
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| Authors: | Chakrabarti R Walker J M Chapman E G Shepardson S P Trdan R J Curole J P Watters G T Stewart D T Vijayaraghavan S Hoeh W R |
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| Affiliation: | Department of Biochemistry, State University of New York, Buffalo, NY 14214, USA. rumela.chakrabarti@gmail.com |
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| Abstract: | Our previous study documented expression of a male-transmitted cytochrome c oxidase subunit II protein (MCOX2), with a C-terminus extension (MCOX2e), in unionoidean bivalve testes and sperm mitochondria. Here, we present evidence demonstrating that MCOX2 is seasonally expressed in testis, with a peak shortly before fertilization that is independent of sperm density. MCOX2 is localized to the inner and outer sperm mitochondrial membranes and the MCOX2 antibody's epitope is conserved across >65 million years of evolution. We also demonstrate the presence of male-transmitted mtDNA and season-specific MCOX2 spatial variation in ovaries. We hypothesize that MCOX2 plays a role in reproduction through gamete maturation, fertilization and/or embryogenesis. |
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| Keywords: | Cytochrome c oxidase subunit II COX2 DUI C-terminus extension mtDNA Male-transmitted mitochondria Unionoidea |
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