Polyploidization in leaf callus tissue and in regenerated plants of dihaploid potato |
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Authors: | E Jacobsen |
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Institution: | (1) Max-Planck-Institut für Züchtungsforschung (Abteilung Straub), Cologne, Germany;(2) Department of Genetics, Biological Centre, University of Groningen, PO Box 14, 9755 AA Haren, The Netherlands |
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Abstract: | Cytological studies on leaf callus cells and regenerated potato plants suggest that it may be possible to utilize somatic
chromosome doubling to obtain tetraploids from outstanding dihaploid breeding clones. The ploidy levels found in callus-derived
plants were diploid, tetraploid, and octaploid, but the proportion of these was dependent on the donor genotype. L1 and L3 germ layers were studied in more than 300 plants; periclinal ploidy chimerism, an undesirable feature of colchicine doubling,
was not found.
Leaf callus was more efficiently induced using NAA than 2, 4-D as an auxin source in the Murashige and Skoog medium. A high
proportion of dividing cells in young calli were polyploid. The frequency of doubled and octaploid plants regenerated was
significantly dependent on donor genotype. The extent of polyploidization was marginally higher after callus growth on a medium
containing 2, 4-D than in a medium containing NAA. In some genotypes the chromosome numbers of regenerated plants were variable,
being less than tetraploid (mixohypotetraploid). After tuber propagation, the original ploidy level was maintained although
mixohypotetraploidy persisted.
In a few somatically doubled clones, male fertility was tested and found to be satisfactory with respect to seed-setting. |
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Keywords: | dihaploid potato somatic doubling tissue culture mixohypotetraploidy |
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