猪和牛轮状病毒抗原和抗体的检测

应用ELISA直接双抗体夹心法检查轮状病毒抗原,24份仔猪和29份犊牛的腹泻粪样,分别有12和16份阳性。用病毒RNA电泳分析检查阳性粪样,各出现两种病毒RNA电泳型,用中和试验检查17份成年牛和16份成年猪血清,分别有16和15份病毒抗体阳性。将其与ELISA间接法和结合法进行了比较。

THE DETECTION OF PORCINE AND BOVINE ROTAVIRUS ANTIGEN AND ANTIBODY

An ELISA direct sandwich assay was developed to detect porcine and bovine rotavirus antigen in feces. 12 were positive in 24 specimens from piglets with diarrhea and 16 were positive in 29 specimens from calves with diarrhea. A comparision of ELISA direct sandwich assay and immune diffusion test for detecting rotavirus antigen in feces showed that the ELISA was more sensitive.ELISA binding assay and neutralization test were used to detect rotavirus antibody in sera, 16 were positive in 17 sera from healthy adult cows in ELISA binding assay and neutralization test and 15 were positive in 16 sera from healthy adult pigs in neutralization test.An ELISA indirect assay was developed and compared with ELISA binding assay in detecting rotavirus antibody. The result showed that the ELISA indirect assay was a little less sensitive than ELISA binding assay, but was more suitable for examining a large number of serum samples.RNA electrophoresis anslysis indicated that there were distinct differences in electrophoresis profiles between porcine and bovine rotaviruses, mainly in band 5 and 6 which were resolved more widely in bovine rotaviruses than in porcine rotaviruses,which might be regarded as characteristic. Two kinds each of eletrophoresis profiles appeared both in porcine and bovine rotaviruses.From the results mentioned above, it is concluded that rotavirus infection is prevalent in some pig and cow farms in Beijing.