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Methionine induction of ACV synthetase inCephalosporium acremonium |
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| Authors: | Jin-you Zhang Gerald Banko Saul Wolfe Dr Arnold L Demain |
| Institution: | (1) Fermentation Microbiology Laboratory, Department of Applied Biological Sciences, Massachusetts Institute of Technology, 02139 Cambridge, MA, USA;(2) Department of Chemistry, Queens University, Kingston, Ontario, Canada;(3) Present address: Chemie Linz AG, A-4020 Linz, Austria |
| Abstract: | Summary Methionine markedly stimulates the biosynthesis of penicillin N and cephalosporin C inCephalosporium acremonium. Examination of intra- and extracellular ACV tripeptide in non-producing mutant N-2 showed that growth in the presence of methionine increased ACV accumulation. Direct measurement of ACV synthetase activity in a cell-free system indicated that the methionine effect was mainly due to induction of this first enzyme of the  -lactam biosynthetic pathway, resulting in a corresponding increase in -lactam production in both a low-producing strain and a high-producing mutant. |
| Keywords: | Methionine effect ACV accumulation |
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