Process optimization for the production of sugar for the bioethanol industry from Tapioca,a non-conventional source of starch |
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Authors: | Aggarwal N.K. Niga P. Singh D. Yadav B.S. |
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Affiliation: | (1) Department of Microbiology, CCS Haryana Agricultural University, Hisar, 125 004, India;(2) School of Biomedical Sciences, University of Ulster, Coleraine, BT 52 1SA Northern Ireland, UK |
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Abstract: | A commercial preparation of -amylase, Biotempase, obtained from Biocon India Pvt. Ltd., and crude glucoamylase produced from Aspergillus sp. NA21 were used to hydrolyse tapioca powder, a non-conventional starchy substrate. Among various concentrations of starch (15–35%, dry weight/volume) tried for maximum liquefaction; slurry made with 25% substrate concentration proved optimal. An economical process of liquefaction was carried out using steam under pressure (0.2–0.3 bar, 104–105 °C) to liquefy a 25% slurry in just 45 min, contrary to a slower process carried out at 95 °C in a water bath. For liquefaction of starch a pH of 5.0 proved to be optimum. The dose of Biotempase as prescribed by the supplier could be reduced by 33% achieving the same degree of liquefaction, by addition of CaCl2 to the starch slurry at the concentration of 120 mg/l. The conditions for the saccharification of liquefied starch were optimized to be 60 °C and pH 5.0, producing 90% saccharification in 24 h. Supplementation of divalent ions Ca2+, Mg2+ and Zn2+ in the process of saccharification showed no effect. Finally glucose was found to be the main hydrolysis product in the saccharification of tapioca starch. |
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Keywords: | /content/x361144763737252/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >-Amylase glucoamylase liquefaction saccharification starch tapioca |
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