Rapid monitoring of recombinant protein products: a comparison of current technologies |
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Authors: | Baker Kym N Rendall Mark H Patel Ashvin Boyd Paul Hoare Mike Freedman Robert B James David C |
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Affiliation: | Research School of Biosciences, University of Kent, Canterbury, UK. |
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Abstract: | Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The choice of product-monitoring technology for a particular bioprocess will depend on a variety of assay factors and instrument-specific factors. In this article, we have compiled an overview of the advantages and disadvantages of the most commonly used technologies used: electrochemiluminescence, optical biosensors, rapid chromatography and nephelometry. The advantages of each technology for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique. |
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