Organic acid metabolism under different glucose concentrations of Leuconostoc oenos from wine |
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Authors: | FM Sagui MC Manca de Nadra |
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Institution: | Càtedra de Microbiología General, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán;Centro de Referencia para Lactobacilos (CERELA), San Miguel de Tucumán, Argentina |
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Abstract: | Leuconostoc oenos M isolated from wine did not grow in the absence of glucose and it was clearly stimulated by the presence of L-malic and citric acids in synthetic medium with different glucose concentrations. In basal medium, D-glucose and L-malic and citric acids were simultaneously consumed. L-Malic acid was metabolized at a higher rate than glucose and citric acid. When the organic acids were completely consumed only 50% of the glucose was utilized. In basal medium 1 mmol of D-lactic acid was produced per mmol of glucose consumed and the amount of ethanol formed was higher with acetate present in the medium. L-Malic acid was completely recovered as L-lactic acid, and in the presence of L-malic acid a carbon imbalance from glucose to D-lactic acid was observed. In the presence of citric acid the amount of D-lactic acid formed was directly proportional to glucose-citrate utilization and acetic acid and ethanol were produced. |
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