The use of phenylmethylsulfonyl fluoride in the study of catabolite inactivation and repression in intact cells of Saccharomyces cerevisiae |
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Authors: | Manfred Klaus Grossmann |
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Affiliation: | (1) Institut für Mikrobiologie, Schnittspahnstraße 10, D-6100 Darmstadt, Federal Republic of Germany |
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Abstract: | Catabolite inactivation of fructose-1,6-bisphosphatase, isocitrate lyase, phosphoenolpruvate carboxykinase and malate dehydrogenase in intact cells could be prevented by phenylmethylsulfonyl fluoride added 40 min prior to the addition of glucose. Protein synthesis, fermentative and respiratory activity and catabolite repression were not affected. Elimination of catabolite inactivation by the addition of PMSF revealed that catabolite repression started at different times for different enzyme.Abbreviation PMSF phenylmethylsulfonyl fluoride |
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Keywords: | Saccharomyces cerevisiae Catabolite repression and inactivation Inhibition of protease B |
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