In Vivo Measurement of Dopamine and Its Metabolites by Intracerebral Dialysis: Changes After d-Amphetamine |
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| Authors: | T Zetterström T Sharp C A Marsden U Ungerstedt |
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| Institution: | Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden;Department of Physiology and Pharmacology, Medical School, Queen's Medical Centre, Nottingham, U.K. |
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| Abstract: | Abstract: By using a new technique, intracerebral dialysis, in combination with high performance liquid chromatography and electrochemical detection, it was possible to recover and measure endogenous extracellular dopamine, together with its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the striatum and nucleus accumbens of anaesthetized or freely moving rats. In addition, measurements of extracellular 5-hydroxyindoleacetic acid, ascorbic acid, and uric acid were made. Basal extracellular concentrations of dopamine and DOPAC in the striatum were estimated to be 5 × 10?8 M and 5 × 10?6 M , respectively. d -Amphetamine (2 mg/kg s.c.) increased dopamine levels in the striatum perfusates by 14-fold, whereas levels of DOPAC and HVA decreased by 77% and 66%, respectively. |
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| Keywords: | In vivo release Brain dialysis Dopamine DOPAC HVA Ascorbic acid d-Amphetamine |
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