Development of a process for the production of the anticancer lead compound pleurotin by fermentation of Hohenbuehelia atrocaerulea |
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Authors: | S M Shipley A L Barr S J Graf R P Collins T G McCloud D J Newman |
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Institution: | Natural Products Support Group, SAIC-Frederick, Inc, Bldg. 431, Box B, Frederick, MD 21702, USA. |
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Abstract: | Pleurotin is a naphthoquinone antibiotic originally isolated from Pleurotus griseus. Two pleurotin producing strains of Hohenbuehelia atrocaerulea have been identified, which, on solid substrate fermentation for 2 months yield 1-2 mg/l of the antibiotic. Described here is the lengthy developmental process which resulted in a production protocol being developed which reliably yields pleurotin from liquid fermentation at >300 mg/l. Critical to obtaining this increase in titer was inclusion in the media of an aqueous extract of alder wood. |
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Keywords: | Pleurotin Hohenbuehelia atrocaerulea |
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