The Structure of the Vacuolar ATPase in Neurospora crassa |
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Authors: | Emilio Margolles-Clark Karen Tenney Emma Jean Bowman Barry J Bowman |
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Institution: | (1) Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz, CA, 95064 |
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Abstract: | The filamentous fungus Neurospora crassa contains many smallvacuoles. These organelles contain high concentrations of polyphosphates andbasic amino acids, such as arginine and ornithine. Because of their size anddensity, the vacuoles can be separated from other organelles in the cell. TheATP-driven proton pump in the vacuolar membrane is a typical V-type ATPase.We examined the size and structure of this enzyme using radiationinactivation and electron microscopy. The vacuolar ATPase is a large andcomplex enzyme, which appears to contain at least thirteen different types ofsubunits. We have characterized the genes that encode eleven of thesesubunits. In this review, we discuss the possible function and structure ofthese subunits. |
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Keywords: | ATPase V-ATPase vacuolar ATPase vacuole fungi neurospora proton pump |
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