Estimation of drug-protein binding parameters on assuming the validity of thermodynamic equilibrium |
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Authors: | Soltés Ladislav Mach Mojmír |
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Affiliation: | Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava. exfasolt@savba.sk |
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Abstract: | This contribution focuses the reader's attention on the pitfalls usually emerging during the phase of evaluation of experimental data of drug-protein binding studies. To overcome the occurrence of problem(s) apparently defying solution, the concept of "affinity spectra" is recommended to be implemented for data evaluation. A (general) "binding study protocol" is also suggested, which can prevent the formation of inadequate conclusions and the generation of unrealistic drug-protein binding parameters. |
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Keywords: | Drug–protein binding Thermodynamic equilibrium Bjerrum plot Reviews |
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