Mapping of the constitutive lysyl-tRNA synthetase gene of Escherichia coli K-12. |
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| Authors: | R V Emmerich and I N Hirshfield |
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| Affiliation: | Department of Biological Sciences, St. John's University, Jamaica, New York 11439. |
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| Abstract: | The constitutive lysyl-tRNA synthetase gene (lysS) was mapped at 62.1 min on the Escherichia coli chromosome by a combination of conjugation and transduction, with physical confirmation by two-dimensional gel electrophoresis. Revertant analysis suggests that the altered isoelectric point and the low amount of the mutant LysS protein may be due to a single mutational event. |
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