| 两种测试方法对DPPH分光光度法测试结果的影响 |
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| 引用本文： | 李小飞,张伟,薛淑媛,李文文,李冠.两种测试方法对DPPH分光光度法测试结果的影响[J].生物技术,2007,17(4):51-53. |
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| 作者姓名： | 李小飞 张伟 薛淑媛 李文文 李冠 |
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| 作者单位： | 新疆大学生命科学与技术学院,新疆,乌鲁木齐,830046 |
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| 基金项目： | 新疆维吾尔自治区高技术项目 |
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| 摘 要： | 目的:比较固定反应时间法和动力学监测法的准确性。方法:用固定反应时间法和动力学监测法分别测定维生素C(Vc)、二丁基羟基甲苯(BHT)对二苯代苦味酰自由基(DPPH)的清除作用,通过F检验和t检验来揭示两种方法的差异。结果:通过动力学监测法得到的测试结果更能准确的反映测试样品对DPPH自由基的清除能力。结论:建议在用DPPH分光光度法过程中使用动力学监测法。
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| 关 键 词： | 固定反应时间法 动力学监测法 DPPH分光光度法 |
| 文章编号： | 1004-311X（2007）04-0051-03 |
| 收稿时间： | 2007-04-18 |
| 修稿时间： | 2007-05-17 |
Effects of Two Test Methods on the Test Result of DPPH Spectrophotometric Assay |
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| LI Xiao-fei,ZHANG Wei,XUE Shu-yuan,LI Wen-wen,LI Guan.Effects of Two Test Methods on the Test Result of DPPH Spectrophotometric Assay[J].Biotechnology,2007,17(4):51-53. |
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| Authors: | LI Xiao-fei ZHANG Wei XUE Shu-yuan LI Wen-wen LI Guan |
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| Institution: | College of Life Science and Technology, Xinjiang University, Urumuqi 830046, China |
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| Abstract: | Objective: To compare the accuracy of reaction time means and kinetic monitoring means.Methods: To determine the 1,1-diphenyl-2-piciylhydrazyl(DPPH) free radical scanvaging activities of vitamin C(Vc) and butylated hydroxyltoluene(BHT) via fixed reaction time means and kinetic monitoring means respectively,and compare the discrepancy of two test means via F-test and t-test.Results:The test result from method of kinetic monitoring can truely show the DPPH radical scanvaging activities of the samples.Conclusion:It is suggested that we should use kinetic monitoring means when use DPPH spectrophotometric assay. |
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| Keywords: | fixed reaction time methods kinetic monitoring means DPPH Spectropho-tometric Assay |
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