Viability and release of complexing compounds during accumulation of heavy metals by a brewer's yeast |
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Authors: | E Soares A Duarte R Boaventura H Soares |
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Institution: | CIEA-Departamento de Engenharia Química, Instituto Superior de Engenharia do Instituto Politécnico do Porto, Rua Dr António Bernardino de Almeida, 431, 4200-072 Porto, Portugal. evsoares@deq.isep.ipp.pt |
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Abstract: | Saccharomyces cerevisiae NCYC 1190 cells accumulated (after 1 h) lead and cadmium at similar levels, and to a lesser degree also copper. During heavy metal accumulation, there was a considerable loss of viability of copper-treated cells (about 99% in the first 20 min of contact with the metal), and a less pronounced lethal effect on cadmium- and lead-treated cells (about 66% and 46% after 1 h of contact with cadmium or lead, respectively) was detected. During copper accumulation, a leakage of UV-absorbing compounds and inorganic phosphate was observed; this did not occur with lead, whereas with cadmium a small amount of leakage of inorganic phosphate was detected. The filtrates of copper-treated cells contained copper-binding molecules. The copper-binding capacity of the filtrates increased with time according to the release of inorganic phosphate and UV-absorbing compounds. These compounds can bind an appreciable quantity of metal ions, making them unavailable for cell uptake and thus reducing the efficiency of heavy metals removal by yeast cells. |
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