Interaction between Paracoccidioides
brasiliensis conidia and the coagulation system: involvement of
fibrinogen |
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Authors: | Diana Tamayo Orville Hernández Cesar Mu?oz-Cadavid Luz Elena Cano Angel González |
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Affiliation: | 1.Molecular and Cell Biology Unit;2.Facultad de Ciencias de la Salud, Institución Universitaria Colegio Mayor de Antioquia, Medellín, Colombia;3.Medical and Experimental Mycology Unit, Corporación para Investigaciones Biológicas, Medellín, Colombia;4.Escuela de Microbiología;5.Basic and Applied Microbiology Research Group, Universidad de Antioquia, Medellín, Colombia |
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Abstract: | The infectious process starts with an initial contact between pathogenand host. We have previously demonstrated that Paracoccidioidesbrasiliensis conidia interact with plasma proteins includingfibrinogen, which is considered the major component of the coagulation system.In this study, we evaluated the in vitro capacity of P.brasiliensis conidia to aggregate with plasma proteins andcompounds involved in the coagulation system. We assessed the aggregation ofP. brasiliensis conidia after incubation with human serumor plasma in the presence or absence of anticoagulants, extracellular matrix(ECM) proteins, metabolic and protein inhibitors, monosaccharides and othercompounds. Additionally, prothrombin and partial thromboplastin times weredetermined after the interaction of P. brasiliensis conidiawith human plasma. ECM proteins, monosaccharides and human plasma significantlyinduced P. brasiliensis conidial aggregation; however,anticoagulants and metabolic and protein inhibitors diminished the aggregationprocess. The extrinsic coagulation pathway was not affected by the interactionbetween P. brasiliensis conidia and plasma proteins, while theintrinsic pathway was markedly altered. These results indicate that P.brasiliensis conidia interact with proteins involved in thecoagulation system. This interaction may play an important role in the initialinflammatory response, as well as fungal disease progression caused byP. brasiliensis dissemination. |
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Keywords: | Paracoccidioides brasiliensis conidia fibrinogen coagulation system |
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