Novel methicillin-resistant coagulase-negative Staphylococcus clone isolated from patients with haematological diseases at the Blood Bank Centre of Amazon,Brazil |
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Authors: | Cristina Motta Ferreira Felipe Gomes Naveca William Antunes Ferreira Cíntia Mara Costa de Oliveira Maria das Gra?as Vale Barbosa |
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Institution: | 1.Fundação Estadual de Hematologia e Hemoterapia do Amazonas, Manaus, AM, Brasil;2.Universidade do Estado do Amazonas, Manaus, AM, Brasil;3.Instituto Leônidas e Maria Deane, Fiocruz, Manaus, AM, Brasil;4.Fundação de Dermatologia Tropical e Venereologia Alfredo da Matta, Manaus, AM, Brasil;5.Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, AM, Brasil |
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Abstract: | Methicillin-resistant Staphylococcus remains a severe public health problem worldwide. This research was intended to identify the presence of methicillin-resistant coagulase-negative staphylococci clones and their staphylococcal cassette chromosome mec (SCCmec)-type isolate from patients with haematologic diseases presenting bacterial infections who were treated at the Blood Bank of the state of Amazonas in Brazil. Phenotypic and genotypic tests, such as SCCmec types and multilocus sequence typing (MLST), were developed to detect and characterise methicillin-resistant isolates. A total of 26 Gram-positive bacteria were isolated, such as: Staphylococcus epidermidis (8/27), Staphylococcus intermedius (4/27) and Staphylococcus aureus (4/27). Ten methicillin-resistant staphylococcal isolates were identified. MLST revealed three different sequence types: S. aureus ST243, S. epidermidis ST2 and a new clone of S. epidermidis, ST365. These findings reinforce the potential of dissemination presented by multi-resistant Staphylococcus and they suggest the introduction of monitoring actions to reduce the spread of pathogenic clonal lineages of S. aureus and S. epidermidis to avoid hospital infections and mortality risks. |
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Keywords: | SCCmec ST clones coagulase-negative Staphylococcus |
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