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Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis
Authors:Tiane Martin de Moura  Ana Paula Vaz Cassenego  Fabrício Souza Campos  Andrea Machado Leal Ribeiro  Ana Cláudia Franco  Pedro Alves d'Azevedo  Jeverson Frazzon  Ana Paula Guedes Frazzon
Abstract:Here we report the presence and expression levels of the vanC1 and vanC 2/3 genes in vancomycin-susceptible strains of Enterococcusfaecalis. The vanC 1 and vanC 2/3 genes were located in the plasmid DNA and on the chromosome,respectively. Specific mRNA of the vanC 1 gene was detected in one of these strains. Additionally, analysis ofthe vanC gene sequences showed that these genes are related tothe vanC genes of Enterococcus gallinarum andEnterococcus casseliflavus. The presence ofvanC genes is useful for the identification of E.gallinarum and E. casseliflavus. Moreover, this isthe first report of vanC mRNA in E.faecalis.
Keywords:Enterococcus faecalis   vancomycin resistance   vanC gene   horizontal gene transfer
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