首页 | 本学科首页   官方微博 | 高级检索  
     


Carnitine requirement of vascular endothelial and smooth muscle cells in imminent ischemia
Authors:W. C. Hülsmann  M. L. Dubelaar
Affiliation:(1) Department of Neurochemistry, National Institute of Mental Health & Neuro Sciences, PB No. 2900, 560 029 Bangalore, India;(2) Department of Neurosurgery, National Institute of Mental Health & Neuro Sciences, 560 029 Bangalore, India
Abstract:Rats were subjected to bilateral carotid artery occlusion for 30 min, followed by reperfusion for varying time periods. The concentration of reduced and oxidized glutathione, glutathione peroxidase and glutathione reductase were determined in whole brain after varying periods of reperfusion. Lipid peroxidation was also assessed by determining the levels of malondialdehyde (MDA) in the brain. Reperfusion for 1 hr following bilateral carotid artery occlusion resulted in significant decrease in total glutathione (GSH) concentration along with small but significant increase in oxidized glutathione (GSSG) levels. After 4 hr of reperfusion, GSH levels recovered, although GSSG levels remained elevated up to 12 hr of reperfusion. Increase in malondialdehyde levels was also detected in the brain up to 12 hr of reperfusion. Glutathione reductase activity remained significantly low up to 144 hr of reperfusion, while glutathione peroxidase activity remained unaffected. These results demonstrate that oxidative stress is generated in the brain during reperfusion following partial ischemia due to bilateral carotid artery occlusion.
Keywords:ischemia  reperfusion  brain  glutathione  glutathione reductase  glutathione peroxidase  lipid peroxidation
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号