Recombinant luciferase-expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors |
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Authors: | He Ran Sandford Gordon Hayward Gary S Burns William H Posner Gary H Forman Michael Arav-Boger Ravit |
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Affiliation: | Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. |
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Abstract: | Recombinant Towne CMV expressing luciferase under the control of CMV-DNA polymerase (POL) or the late pp28 (UL99) promoters were evaluated for potential application in high-throughput screening of anti-viral compounds. POL-and pp28-luciferase displayed maximal expression 48 and 72 hours post infection, respectively. The pp28-luciferase virus achieved a wider dynamic range of luciferase expression (6-7 logs) and was selected for testing of inhibition by five anti-viral compounds. Luciferase expression highly correlated with plaque reduction and real-time PCR. The pp28-luciferase reporter system is rapid, reproducible, and highly sensitive. It may be applied to screening of novel anti-CMV compounds. |
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