Informational content of mitochondrial DNA from a "low density" petite mutant of yeast |
| |
Authors: | F Carnevali C Falcone L Frontali L Leoni G Macino C Palleschi |
| |
Affiliation: | Centro di Studio per gli Acidi Nucleici del C.N.R., Istituto di Fisiologia Generale, Università di Roma Italy |
| |
Abstract: | Mitochondrial DNA from wild-type yeast contains the genetic information for some mitochondrial tRNAs including tRNAser and tRNAphe. In a “low density” petite mutant, mitochondrial DNA still retains the information for tRNAser, while the information for tRNAphe is lost.The permanence of genetic information in this DNA containing only 3.6% G+C supports previous results concerning its intramolecular heterogeneity. An irregular distribution of G+C content along the molecule was further demonstrated by annealing experiments performed with DNA fragmented by sonication and fractionated on CsCl density gradient. These experiments show that the heavy fractions of the gradient preferentially anneal with mitochondrial seryl-tRNA. |
| |
Keywords: | mit-DNA mitochondrial DNA w.t. wild type A adenine T thymine G guanine C cytosine SSC standard saline-citrate EDTA ethylenediaminetetracetate |
本文献已被 ScienceDirect 等数据库收录! |