青岛、淄博地区人轮状病毒分子流行病学研究

用核酸凝胶电泳法对1983～1984年青岛、淄博地区的108份轮状病毒标本作了RNA电泳型分析。发现同一时期两地区流行的轮状病毒亚群不同,同一时期存在两亚群毒株的共同流行。共检出13个不同的差异电泳型,其中4个电泳型多于11条核酸带。提示轮状病毒毒株间存在一定的变异,电泳型不同的毒株可能同时感染一个病人。本文还对轮状病毒核酸电泳型多形性的原因及意义进行了讨论。

MOLECULAR EPIDEMIOLOGY OF HUMAN ROTAVIRUS IN QINGDAO AND ZIBO FROM 1983-1984

Using the method of PAGE,we analyzed the electrophoretype of RNA rota-virus obtained from 108 stool samples with acute infantile diarrhea in Qing-dao and Zibo. Different subgroups of human rotavirus(HRV) prevailed in the two areas that are very near in their geographical position. In 1983-1984, subgroup 11 was found to be the main etiological agent in Qingdao. In contrast, subgroup I was found to be the main etiological agent in Zibo during the same period.In total, 13 different variant electrophoretypes were observed.9 of them belonged to the long electrophoretype while 4 conformed to the short. Among the "long", 4 consisted of more than 11 bands, suggesting simultaneous infection by more than one types in the same patient.