Identification of an Escherichia coli inner membrane polypeptide specified by a lambda-tonB transducing. |
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Authors: | G S Plastow I B Holland |
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Institution: | Department of Genetics, The University of Leicester, University Road, Leicester LE1 7RH, England |
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Abstract: | Analysis by polyacrylamide gel electrophoresis of the proteins coded by a λB transducing phage, after infection of UV-irradiated bacteria, revealed the presence of at least 7 new polypeptides. Three of these were identified as proteins of the operon whilst three others were deleted by a spontaneous mutation in the B region carried by the phage. A single polypeptide, molecular weight 40,000 was absent from a phage carrying a proflavine induced mutation in B. We conclude that this protein, which was localised in the inner membrane by sarkosyl fractionation of the envelope, is the product. |
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Keywords: | SDS sodium dodecyl sulphate PAGE polyacrylamide gel electrophoresis kd kilodaltons |
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