The content and chain length of polyphosphates from vacuoles of Saccharomyces cerevisiae VKM Y-1173 |
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Authors: | Trilisenko L V Vagabov V M Kulaev I S |
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Institution: | (1) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia |
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Abstract: | The content of inorganic polyphosphates (polyP) in vacuoles of the yeast Saccharomyces cerevisiae is 15% of the total cellular polyP. Over 80% of the vacuole polyP are in an acid-soluble fraction. It was first established by 31P-NMR spectroscopy that a polymeric degree (n) of two subfractions obtained by precipitation with Ba2+ in succession at pH 4.5 and 8.2 was approximately 20 ± 5 and 5 ± 2 residues of ortho-phosphoric acid, respectively. Under a deficit of phosphate (Pi) in the cultivation medium, the polyP content in vacuoles decreased 7-fold with the same drastic reduction of their content in the cell. Unlike intact yeast cells, where polyP overcompensation is observed after their transfer from phosphate-free to phosphate-containing medium, the vacuoles do not show this effect. The data indicate the occurrence of special regulatory mechanisms of polyP synthesis in vacuoles differing from those in the whole cell. |
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Keywords: | yeast protoplasts vacuoles overcompensation polyphosphates exopolyphosphatase |
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