Cell elongation is an adaptive response for clearing long chromatid arms from the cleavage plane |
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Authors: | Shaila Kotadia Emilie Montembault William Sullivan Anne Royou |
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Institution: | 1.Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064;2.Université de Bordeaux, Institut Européen de Chimie et Biologie/Institut de Biochimie et Génétique Cellulaire (IECB/IBGC), UMR 5095, 33607 Pessac, France;3.Centre National de la Recherche Scientifique, IECB/IBGC, UMR 5095, 33607 Pessac, France |
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Abstract: | Chromosome segregation must be coordinated with cell cleavage to ensure correct transmission of the genome to daughter cells. Here we identify a novel mechanism by which Drosophila melanogaster neuronal stem cells coordinate sister chromatid segregation with cleavage furrow ingression. Cells adapted to a dramatic increase in chromatid arm length by transiently elongating during anaphase/telophase. The degree of cell elongation correlated with the length of the trailing chromatid arms and was concomitant with a slight increase in spindle length and an enlargement of the zone of cortical myosin distribution. Rho guanine-nucleotide exchange factor (Pebble)–depleted cells failed to elongate during segregation of long chromatids. As a result, Pebble-depleted adult flies exhibited morphological defects likely caused by cell death during development. These studies reveal a novel pathway linking trailing chromatid arms and cortical myosin that ensures the clearance of chromatids from the cleavage plane at the appropriate time during cytokinesis, thus preserving genome integrity. |
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