Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast |
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| Authors: | Junqi Huang Yinyi Huang Haochen Yu Dhivya Subramanian Anup Padmanabhan Rahul Thadani Yaqiong Tao Xie Tang Roland Wedlich-Soldner Mohan K Balasubramanian |
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| Institution: | 1.Cell Division Laboratory, Temasek Life Sciences Laboratory, Singapore 117604;2.Department of Biological Sciences, National University of Singapore, Singapore 117543;3.Mechanobiology Institute, Singapore 117411;4.Cellular Dynamics and Cell patterning Research Group, Max Planck Institute of Biochemistry, Martinsried 82152, Germany |
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| Abstract: | In many eukaryotes, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring. Despite the central role of this ring in cytokinesis, the mechanism of F-actin assembly and accumulation in the ring is not fully understood. In this paper, we investigate the mechanism of F-actin assembly during cytokinesis in Schizosaccharomyces pombe using lifeact as a probe to monitor actin dynamics. Previous work has shown that F-actin in the actomyosin ring is assembled de novo at the division site. Surprisingly, we find that a significant fraction of F-actin in the ring was recruited from formin-Cdc12p nucleated long actin cables that were generated at multiple nonmedial locations and incorporated into the ring by a combination of myosin II and myosin V activities. Our results, together with findings in animal cells, suggest that de novo F-actin assembly at the division site and directed transport of F-actin cables assembled elsewhere can contribute to ring assembly. |
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