Enhanced conversion of sucrose to isomaltulose by a mutant of Erwinia rhapontici |
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Authors: | Ahn Seung-Joon Yoo Ji-Hyun Lee Hyeon-Cheol Kim Sang-Yong Noh Bong-Soo Kim Jung-Hoe Lee Jung-Kul |
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Institution: | (1) BioNgene Co., Ltd, 10-1, 1Ka, Myungryun-Dong, Jongro-Ku, Seoul, 110-521, Korea;(2) Department of Food & Microbial Technology, Seoul Women's University, 126, Kongnung 2-Dong, Nowon-Ku, Seoul, 139-774, Korea;(3) Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-Dong, Yuseong-Ku, Daejeon, 305-701, Korea |
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Abstract: | Mutagenesis of Erwinia rhapontici was performed to enhance the production of isomaltulose from sucrose. A mutant strain, BN 68089, was obtained through a screening process involving automated and miniaturized cultivation in Bioscreen C. This high-throughput, miniaturized screening system was optimized to identify the mutant strain, which had a conversion yield (90%) and productivity (194 g l–1 h–1). The BN 68089 mutant cells were immobilized in sodium alginate and when operated in a packed bed reactor gave a yield of 89% and a productivity of 144 g l–1 h–1 of at 30 °C, the optimal temperature. Immobilized BN 68089 cells exhibited 8% and 15% higher yield and productivity, respectively, than those of the wild-type strain. |
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Keywords: | Bioscreen C Erwinia rhapontici high-throughput screening immobilization isomaltulose |
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