拟南芥砷诱导基因At4g13180的表达模式研究

拟南芥(Arabidopsis thaliana)砷诱导基因At4g13180编码蛋白是短链脱氢酶(Short-Chain Dehydrogenase/Reductase Superfamily,SDR)家族的成员之一,其过表达可以增强植物对过氧化氢的耐受性。该实验通过半定量RT-PCR,构建ProAt4g13180:GUS、At4g13180-EGFP和At4g13180-OE表达载体,获得At4g13180基因过表达转基因株系,并研究了At4g13180基因的表达模式及其编码蛋白的亚细胞定位。结果显示,At4g13180基因在根尖、叶脉、萼片和花丝等组织都强烈表达,该基因编码蛋白主要定位于胞质和核中。该研究结果为深入探究拟南芥砷诱导基因At4g13180的功能奠定了一定的基础。

Expression Pattern Analysis of Arabidopsis thaliana Arsenic Induced Gene At4g13180

In Arabidopsis thaliana, the arsenic induced gene At4g13180 encoding protein is one of the members of the SDR superfamily (Short Chain Dehydrogenase/Reductase). Its overexpression can enhance the resistance of plants to hydrogen peroxide. Here we studied the At4g13180 gene expression pattern and the encoded protein subcellular localization by semi quantitative RT PCR, with transgenic plants expressing ProAt4g13180:GUS, At4g13180 EGFP and At4g13180 OE. Our results show that the At4g13180 gene has a very strong expression in tissues such as root, leaf, sepal, and stamen filamentas. At4g13180 EGFP is located in the nucleus and cytoplasm. Our study lays a basis of further research on the role of the arsenic induced gene At4g13180 in A. thaliana.