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Entamoeba histolytica: specific antigen recognized by a monoclonal antibody |
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| Authors: | L Ortiz-Ortiz C Ximénez F Mendoza C Michalak E I Melendro A Oliva |
| Affiliation: | 2. Department of Immunology, Institutc de Investigations Biomédicas, Universidad National Autónoma de México, 04510 México, D. F., Mexico;1. Department of Experimental Medicine, Facultad de Medicina, Universidad National Autónoma de México, 04510 México, D. F., Mexico;1. Myanmar–Oxford Clinical Research Unit, Yangon, Myanmar;2. Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK;3. Shoklo Malaria Research Unit, Mae Sot, Thailand;4. Mahidol–Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;1. Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., Ciudad de México, Mexico;2. Programa de Doctorado en Ciencias Biomédicas, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de, Mexico |
| Abstract: | Specific antigenic determinants on the membrane surface of Entamoeba histolytica that distinguish it from other Entamoeba species were demonstrated. Evidence for these antigenic determinants was obtained with a monoclonal antibody to E. histolytica which showed not only specificity but also sensitivity as demonstrated in enzyme linked immunosorbent assay. Immunofluorescence microscopy showed that the monoclonal antibody recognized an epitope present on the membrane surface of E. histolytica trophozoites. The epitope detected by the monoclonal antibody was present in three components of different molecular weight. These components may have a common precursor or may be the result of enzymatic degradation under the conditions tested. |
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