A micropropagation protocol for <Emphasis Type="Italic">Cassia angustifolia</Emphasis> Vahl. from root explants |
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Authors: | Shahina Parveen Anwar Shahzad |
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Institution: | (1) Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202002, UP, India; |
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Abstract: | The aim of this study was to develop a new micropropagation system for Cassia angustifolia Vahl., an important medicinal legume using root explant as starting material. Root explants taken from 30-day-old aseptic
seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different plant growth regulators: 6-benzyladenine
(BA), kinetin (Kn), and thidiazuron (TDZ). Organogenic nodular calli obtained on MS + TDZ (1.0 μM) were transferred to shoot
regeneration medium supplemented with different cytokinins (BA, Kn or TDZ) either alone or in combination with auxin:indole-3-acetic
acid or α-naphthalene acetic acid. Maximum shoot regeneration frequency (90%) was obtained on MS + BA (2.5 μM) + NAA (0.6 μM)
wherein a maximum of 42.76 ± 1.47 shoot buds per explant were induced with a maximum conversion rate of 35.63 ± 0.75 shoots
per explant and average shoot length of 5.43 ± 0.20 cm. Elongated microshoots were successfully rooted under ex vitro conditions
by pulse treatment in 200 μM of indole-3-butyric acid for half an hour. Microshoots were rooted, acclimatized and hardened
off simultaneously in sterilized soilrite inside the growth room and then established in pots containing sterilized soil and
manure (1:1) and grown under greenhouse condition with 90% survival rate. The histological sections at different developmental
stages of shoot buds revealed the organization of nodular meristematic zone leading to the orientation and differentiation
of shoot buds in large number and thereafter conversion into healthy shoots. |
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