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Tiba inhibition of <Emphasis Type="Italic">in vitro</Emphasis> organogenesis in excised tobacco leaf explants
Authors:Harbinder?S?Dhaliwal  Edward?C?Yeung  Email author" target="_blank">Trevor?A?ThorpeEmail author
Institution:(1) Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4 Calgary, Alberta, Canada
Abstract:Summary Triiodobenzoic acid (TIBA), an anti-auxin, was found to inhibit both shoot and root formation in cultured excised leaf explants of tobacco (Nicotiana tabacum L.). The shoot formation (SF) medium used required only exogenous cytokinin (N6-benzyladenine) and the root formation (RF) medium required both auxin (indole-3-butyric acid) and cytokinin (kinetin). By transferring the explants from SF or RF media to SF or RF media with TIBA (4.0×10−5 M), respectively or vice versa, at different times in culture, it was found that TIBA inhibition was at the time of meristemoid formation and after determination of organogenesis. This indicates that TIBA interfered with endogenous auxin involvement in organized cell division.
Keywords:anti-auxin  auxin inhibitor  meristemoids  root primordial  shoot primordial  triiodobenzoic acid
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