Area-based analyzing technique at cell array experiment using neuronal cell line |
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Authors: | Shigeru Yamada Eiichiro Uchimura Takanori Ueda Fukumi Iguchi Yutaka Akiyama Satoshi Fujita Masato Miyake Jun Miyake |
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Institution: | (1) Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Nakouji 3-11-46, Amagasaki, 661-0974 Hyogo, Japan |
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Abstract: | We propose a simple procedure for the identification and quantitative analysis of neurite outgrowth in neuronal cell lines
that were uniformly differentiated. Upon stimulation most neuronal cell lines extend neurites in the differentiation process,
resulting, according to our observation, in the increase of cell surface area. This increase is dependent on the length and
the number of extended neurites. Furthermore, we use this method for the phenotype analysis of cell array experiments to perform
large-scale functional evaluation of genes involved in the neurite outgrowth during neuronal differentiation. |
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Keywords: | Cell array high throughput neurite surface area image analysis |
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