Divergent human populations show extensive shared IGK rearrangements in peripheral blood B cells |
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Authors: | Email author" target="_blank">Katherine?Jean?Louise?JacksonEmail author Yan?Wang Bruno?A?Gaeta William?Pomat Peter?Siba Janet?Rimmer William?A?Sewell Andrew?M?Collins |
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Institution: | (1) School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, Australia;(2) School of Computer Science and Engineering, The University of New South Wales, Sydney, NSW, Australia;(3) Papua New Guinea Institute of Medical Research, Goroka, Eastern Highlands Province, Papua New Guinea;(4) St Vincent’s Clinic, Darlinghurst, NSW, Australia;(5) The Garvan Institute of Medical Research and St Vincent’s Clinical School, University of New South Wales, Sydney, NSW, Australia;(6) Present address: Department of Pathology, Stanford University, 300 Pasteur Drive, R214 MC 5324, Stanford, CA 94305, USA |
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Abstract: | We have analysed the transcribed immunoglobulin kappa (IGK) repertoire of peripheral blood B cells from four individuals from
two genetically distinct populations, Papua New Guinean and Australian, using high-throughput DNA sequencing. The depth of
sequencing data for each individual averaged 5,548 high-quality IGK reads, and permitted genotyping of the inferred IGKV and
IGKJ germline gene segments for each individual. All individuals were homozygous at each IGKJ locus and had highly similar
inferred IGKV genotypes. Preferential gene usage was seen at both the IGKV and IGKJ loci, but only IGKV segment usage varied
significantly between individuals. Despite the differences in IGKV gene utilisation, the rearranged IGK repertoires showed
extensive identity at the amino acid level. Public rearrangements (those shared by two or more individuals) made up 60.2%
of the total sequenced IGK rearrangements. The total diversity of IGK rearrangements of each individual was estimated to range
from just 340 to 549 unique amino acid sequences. Thus, the repertoire of unique expressed IGK rearrangements is dramatically
less than previous theoretical estimates of IGK diversity, and the majority of expressed IGK rearrangements are likely to
be extensively shared in individual human beings. |
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