Inhibition of the uptake of 5-hydroxytryptamine, noradrenaline and dopamine into rat brain homogenates by various hydroxylated tryptamines |
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Authors: | A S Hoax H G Baumgarten H G Schlossberger |
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Affiliation: | MRC Neurochemical Pharmacology Unit, Department of Pharmacology, University of Cambridge England;Department of Neuroanatomy, University of Hamburg, 2 Hamburg 20;Max-Planck-Institute of Biochemistry, 8033 Martinsried, Munchen, W. Germany |
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Abstract: | Recent work has shown that intracerebral injections of 5,6-dihydroxytryptamine (5,6-DHT) lead to a fairly selective and long lasting depletion of 5-HT in the rat CNS (BAUMGARTEN, BJORKLUND, LACHENMAYER, NOBIN and STENEVI, 1971; DALY, FUXE and JONSSON, 1973). This effect appears to result from a degeneration of the serotonin-containing neurons (BAUMGARTEN and LACHENMAYER, 1972a). 5,6-DHT does, however, to a lesser extent affect both NA and dopamine (DA) containing nerve terminals (BAUMGARTEN et al., 1971). In an attempt, therefore, to find compounds having a more specific toxic action we have investigated several other hydroxylated tryptamines. In order to obtain information about the differential affinities of these analogues for neuronal uptake sites we have examined their effects on the uptake of 3H]5-HT and (±)-3H]NA into synaptosomes in homogenates of rat hypothalamus and of 3H]DA uptake into a similar preparation from the rat corpus striatum. It is known that the uptake of these putative transmitters in rat brain homogenates is predominantly into the synaptosome fraction (KANNENGIESSER, HUNT and RAYNAUD, 1973; COYLE and SNYDER, 1969). |
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