Changes in the bacterial populations of the highly alkaline saline soil of the former lake Texcoco (Mexico) following flooding |
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Authors: | César Valenzuela-Encinas Isabel Neria-González Rocio J Alcántara-Hernández Isabel Estrada-Alvarado Francisco Javier Zavala-Díaz de la Serna Luc Dendooven Rodolfo Marsch |
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Institution: | (1) Departamento de Biotecnología y Bioingeniería, Cinvestav, Campus Zacatenco, 07360 Mexico, DF, México;(2) Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, Cd. Obregón, 85000 Sonora, México |
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Abstract: | Flooding an extreme alkaline-saline soil decreased alkalinity and salinity, which will change the bacterial populations. Bacterial
16S rDNA libraries were generated of three soils with different electrolytic conductivity (EC), i.e. soil with EC 1.7 dS m−1 and pH 7.80 (LOW soil), with EC 56 dS m−1 and pH 10.11 (MEDIUM soil) and with EC 159 dS m−1 and pH 10.02 (HIGH soil), using universal bacterial oligonucleotide primers, and 463 clone 16S rDNA sequences were analyzed
phylogenetically. Library proportions and clone identification of the phyla Proteobacteria, Actinobacteria, Acidobacteria, Cyanobacteria, Bacteroidetes, Firmicutes and Cloroflexi showed that the bacterial communities were different. Species and genera of the Rhizobiales, Rhodobacterales and Xanthomonadales
orders of the α- and γ-subdivision of Proteobacteria were found at the three sites. Species and genera of the Rhodospirillales, Sphingobacteriales, Clostridiales, Oscillatoriales
and Caldilineales were found only in the HIGH soil, Sphingomonadales, Burkholderiales and Pseudomonadales in the MEDIUM soil,
Myxococcales in the LOW soil, and Actinomycetales in the MEDIUM and LOW soils. It was found that the largest diversity at
the order and species level was found in the MEDIUM soil as bacteria of both the HIGH and LOW soils were found in it. |
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Keywords: | Clone library 16S rDNA Bacterial community Alkaline-saline soil |
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