Adsorption on synthetic membranes and immunogenic properties of aprotinin cross-linked by glutaraldehyde] |
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| Authors: | T A Timofeeva P B Goliando O P Zhirnov |
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| Institution: | Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences, Moscow, Russia. |
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| Abstract: | A procedure for the intermolecular crosslinking of aprotinin (natural polypeptide, an inhibitor of serine proteases) by glutaraldehyde was proposed. This autoconjugation of aprotinin increased its immunogenicity and the efficiency of sorption on nitrocellulose and polyvinylidene difluoride membranes. The immunization of animals by the autoconjugated aprotinin induced the production of antibodies that reacted with both conjugated and monomeric aprotinin. The properties of the crosslinked aprotinin are promising for the enhancement of sensitivity through its use in EIA and immunoblotting membrane methods that employ aprotinin and antiaprotinin antibodies. |
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