若尔盖高原产甲烷菌数量的时空差异性

应用实时荧光定量PCR法,构建了总甲烷菌实时荧光绝对定量PCR的标准品以及标准曲线,用于总甲烷菌的定量测定,所构建的质粒DNA浓度为160mg/L,标准曲线相关性为0.992,扩增效率为98.6%。研究表明,若尔盖花湖地区4月草地生态系统和湿地生态系统甲烷菌含量相当,湿地生态系统9月甲烷菌含量高于7月,7月甲烷菌含量高于4月,在7月和9月随着深度的增加甲烷菌含量也增加,而草地生态系统的甲烷菌含量并未表现出明显的季节和深度上的规律性。

Time-space Change of Methanogen Amount from Zoige Plateau

To quantify the population size of total methanogens changing with environments, season and depths of Zoige plateau, the plasmids and standard curves of species were constructed by using real - time absolute quantitative PCR. The concentration of the standard plasmids was 160 mg/L, with the coefficient being 0.992, and the amplification efficiency was 98.6%. The results showed that in April the population size of total methanogens in wetland ecosystem and grassland ecosystem were nearly the same. In wetland ecosystem, the population size of total methanogens decreased in the order of September, July, and April, while increased with the sampling depth in September and July, In grassland ecosystem, however, the population size of total methanogens did not change significantly with season and depth.