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Hematopoietic organs of<Emphasis Type="Italic"> Manduca sexta</Emphasis> and hemocyte lineages
Authors:Email author" target="_blank">James?B?NardiEmail author  Barbara?Pilas  Elizabeth?Ujhelyi  Karl?Garsha  Michael?R?Kanost
Institution:(1) Department of Entomology and Department of Natural Resources and Environmental Sciences, University of Illinois, 320 Morrill Hall, 505 South Goodwin Avenue, Urbana, IL 61801, USA;(2) Flow Cytometry Facility, Biotechnology Center, University of Illinois, 231 Edward R. Madigan Laboratory, 1201 West Gregory Drive, Urbana, IL 61801, USA;(3) Center for Microscopy and Imaging, College of Veterinary Medicine, University of Illinois, 2001 South Lincoln Avenue, Urbana, IL 61801, USA;(4) Imaging Technology Group, Beckman Institute for Advanced Science and Technology, University of Illinois, 405 N Mathews Avenue, Urbana, IL 61801, USA;(5) Department of Biochemistry, Kansas State University, 104 Willard Hall, Manhattan, KS 66506, USA
Abstract:Cells of the moth immune system are derived from organs that loosely envelop the four wing imaginal discs. The immune response in these insects is believed to depend on the activities of two main classes of hemocytes: plasmatocytes and granular cells. The fates of cells that arise from these hematopoietic organs have been followed by immunolabeling with plasmatocyte-specific and granular-cell-specific antibodies. Cells within each hematopoietic organ differ in their coherence and in their expression of two plasmatocyte-specific surface proteins, integrin and neuroglian. Within an organ there is no overlap in the expression of these two surface proteins; neuroglian is found on the surfaces of the coherent cells while integrin is expressed on cells that are losing coherence, rounding up, and dispersing. A granular-cell-specific marker for the protein lacunin labels the basal lamina that delimits each organ but only a small number of granular cells that lie on or near the periphery of the hematopoietic organ. When organs are cultured in the absence of hemolymph, all cells derived from hematopoietic organs turn out to immunolabel with the plasmatocyte-specific antibody MS13. The circulating plasmatocytes derived from hematopoietic organs have higher ploidy levels than the granular cells and represent a separate lineage of hemocytes.Edited by P. Simpson
Keywords:Hemocytes  Plasmatocytes  Granular cells  Hematopoiesis  Insect immunity
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