Indirect somatic embryogenesis and morphohistological analysis in <Emphasis Type="Italic">Capsicum chinense</Emphasis> |
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Authors: | Laura Y Solís-Ramos Sara Nahuath-Dzib Antonio Andrade-Torres Felipe Barredo-Pool Tomas González-Estrada Enrique Castaño de la Serna |
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Institution: | 1.Unidad de Bioquímica y Biología Molecular de Plantas,Centro de Investigación Científica de Yucatán,Mérida, Yucatán,México;2.Escuela de Biología,Universidad de Costa Rica,San Pedro,Costa Rica;3.Laboratorio de Biotecnología, Departamento de Ingeniería Química y Bioquímica,Instituto Tecnológico de Mérida,Mérida, Yucatán,México;4.INBIOTECA,Universidad Veracruzana,Xalapa, Veracruz,México;5.Unidad de Biotecnología,Centro de Investigación Científica de Yucatán,Mérida, Yucatán,México |
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Abstract: | Capsicum chinense is recalcitrant in in vitro morphogenesis. No efficient, reproducible somatic embryogenesis regeneration system exists for this species, impeding regeneration
from transformed cells. An indirect somatic embryogenesis protocol is developed using mature C. chinense zygotic embryo segments (ZES). The ZES cultured in semi-solid Murashige-Skoog (MS) medium supplemented with 8.9 μM naphthaleneacetic
acid, 11.4 μM indoleacetic acid and 8.9 μM 6-benzylaminopurine, developed an embryogenic callus and 8% of the calli developed
somatic embryos. Torpedo-stage somatic embryos were detached from the callus and subcultured in semi-solid MS medium without
growth regulators, producing a 75% conversion rate to plantlets with well-formed root tissue. Histological analysis showed
the developed structures to have no vascular connection with the callus and to be bipolar, confirming that this protocol induced
formation of viable somatic embryos from mature C. chinense ZES. All acclimated plantlets survived under greenhouse conditions. This protocol will facilitate regeneration of genetically
transformed plants using either biolistics or Agrobacterium tumefaciens approach. |
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