Efficient validation of single nucleotide polymorphisms in plants by allele-specific PCR,with an example from barley |
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| Authors: | V?D?Soleimani Email author" target="_blank">B?R?BaumEmail author D?A?Johnson |
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| Institution: | (1) Agriculture and Agri-Food Canada (AAFC) Central Experimental Farm, K1A 0C6 Ottawa, ON, Canada;(2) Ottawa-Carleton Institute of Biology, University of Ottawa, 30 Marie Curie, K1N 6N5 Ottawa, ON, Canada |
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| Abstract: | Although the increasing number of expressed sequence tags (ESTs) from the public domain has facilitated the detection of single
nucleotide polymorphisms (SNPs), further validation is needed before they can be used as markers. For SNP validation, we have
compared 2 independent methods: (1) the primer extension method followed by capillary electrophoresis on an ABI PRISM 3100
Genetic Analyzer and (2) nested PCR followed by agarose-based visualization. We present an assessment of the efficiency and
costs associated with these methods, based on a sample of barley cultivars. |
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| Keywords: | allele-specific PCR SNP validation |
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